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METHOD FOR PREPARING HUMAN HYBRID CELLS EXPRESSING HETEROLOGOUS PROTEIN, HUMAN HYBRID CELLS STRAIN HKB11, STRAIN OF HUMAN HYBRID CELLS IG2
METHOD FOR PREPARING HUMAN HYBRID CELLS EXPRESSING HETEROLOGOUS PROTEIN, HUMAN HYBRID CELLS STRAIN HKB11, STRAIN OF HUMAN HYBRID CELLS IG2
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机译：制备表达杂种蛋白的人杂种细胞，人杂种细胞株HKB11，人杂种细胞IG2株的方法
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摘要
FIELD: biotechnology. SUBSTANCE: invention concerns human hybrid cells. Hybrid cells of human/human are prepared by fusion of human embryo kidney cells (293S) and Burkitt's lymphoma modified cells. Fusion cells can be used as host-cells for recombinant expression of mammal genes. Method provides preparing human hybrid cells strain HKB1, ATCC:CRL-12568 used as a host-cell for expression of heterologous protein eliciting the human profile of glycosylation reaction. Also, method provides preparing human hybrid cells strain IG2, ATCC:PTA-87 expressing protein IL-4SA (T13D/R121E). Advantages in using these hybrid clones of human kidney cells and B- cells named as HKB are the following: cells show negative property by immunoglobulin expression, they show good growth in medium without plasma proteins (with addition of recombinant insulin or without the latter), as suspension cultures in shaker bottle or in fermenter, they show high sensitivity for transfection with DNA and produce abundant amounts of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4 and rFVIII. EFFECT: improved preparing method. 15 cl, 3 dwg, 4 tbl, 3 ex
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机译：领域：生物技术。物质：本发明涉及人类杂交细胞。人/人杂交细胞是通过人胚肾细胞（293S）和Burkitt淋巴瘤修饰细胞融合而制备的。融合细胞可用作宿主细胞用于哺乳动物基因的重组表达。该方法提供了制备人杂种细胞株HKB1，ATCC：CRL-12568作为宿主细胞用于表达引起人糖基化反应的异源蛋白的表达。同样，该方法提供了制备表达蛋白IL-4SA的人杂种细胞株IG2，ATCC：PTA-87的蛋白（T13D / R121E）。使用这些人类肾脏细胞和B细胞（称为HKB）的杂交克隆的优势如下：细胞通过免疫球蛋白表达表现出阴性特性，它们在不含血浆蛋白（添加重组胰岛素或不含重组胰岛素）的培养基中表现出良好的生长，作为摇瓶或发酵罐中的悬浮培养物，它们显示出对DNA转染的高敏感性，并产生大量异源重组蛋白，例如重组单克隆抗体，可溶性ICAM-1，rIL-4和rFVIII。效果：改进了制备方法。 15 cl，3 dwg，4 tbl，3 ex
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