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METHOD FOR SCREENING COMPOUND BINDING TO THE ACTIVE SITE OF TARGET PROTEIN BY USING PROTEIN LABELED ON SPECIFIC AMINO ACIDS AND 2D NMR TECHNIQUE
METHOD FOR SCREENING COMPOUND BINDING TO THE ACTIVE SITE OF TARGET PROTEIN BY USING PROTEIN LABELED ON SPECIFIC AMINO ACIDS AND 2D NMR TECHNIQUE
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机译：利用特定氨基酸标记的蛋白质和二维核磁共振技术筛选目标蛋白质活性位点的化合物结合方法
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摘要
PURPOSE: A method for screening a compound capable of binding to an active site of a protein using an amino acid-labeled protein and a 2D NMR technique is provided, thereby providing lead compounds for developing new medicines. CONSTITUTION: The method for screening a compound capable of binding to an active site of a protein using an amino acid-labeled protein and a 2D NMR technique comprises the steps of: (1) preparing a certain amino acid-15N or 13C labeled target protein and measuring its 2D NMR spectrum; (2) collecting known compounds, or peptides or peptide analogues capable of binding with the target protein; (3) determining the active site of a protein through binding test between the target protein-active site binding compound, target protein-peptide or target protein-peptide analogues; and (4) screening compounds showing change in the active site signal through the protein-compound binding test, wherein the target protein is an enzyme or a receptor.
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