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METHOD FOR MANUFACTURING RECOMBINANT STRESS-ACTIVATED PROTEIN KINASE-(SAPK-) USING E.COLI
METHOD FOR MANUFACTURING RECOMBINANT STRESS-ACTIVATED PROTEIN KINASE-(SAPK-) USING E.COLI
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机译:大肠杆菌制备重组应激活化蛋白激酶-(SAPK-)的方法
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摘要
PURPOSE: Provided is a method for manufacturing recombinant stress-activated protein kinase-(SAPK-), as one of mitogen-activated protein kinase using E.coli. The recombinant stress-activated protein kinase-(SAPK-) is used for analysis of three-dimensional structure by nuclear magnetic resonance spectroscopy and X-ray crystallization and development of inflammation inhibitor. CONSTITUTION: A method for manufacturing recombinant stress-activated protein kinase-(SAPK-) comprises the steps of: transforming E.coli with an expression vector pRGT7HSAPK to give E.coli transformant BL21(DE3)/pRGT7HSAPK (KCTC 0450BP); culturing the transformant and followed by cytolysis; centrifuging the crude lysate to remove insoluble protein; and purifying SAPK- by chromatography, dialysis and filtration chromatography.
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