首页> 外国专利> Regenerating cassava plants from protoplasts that have been isolated from friable embryogenic callus (FEC)

Regenerating cassava plants from protoplasts that have been isolated from friable embryogenic callus (FEC)

机译:从脆弱的胚性愈伤组织(FEC)中分离出的原生质体再生木薯植物

摘要

A method for producing protoplasts of cassava or closely related species in which the protoplasts are capable of regeneration into plants is described. The method comprises producing friable embryongenic callus from explants of cassava or closely related species and isolating protoplasts from the friable embryongenic cells. Further described is a method for transforming a protoplast of cassava or closely related species and transformed protoplasts obtained thereby. In addition, a method for regenerating plants from these protoplasts is described and a cassava plant or closely related species obtained thereby. Starch isolated from tubers of such cassava plants have an amylopectin content of at least about 90% wt.% based on the dry weight of the starch.
机译:描述了一种生产木薯或紧密相关物种的原生质体的方法,其中原生质体能够再生为植物。该方法包括从木薯或密切相关物种的外植体产生易碎的胚性愈伤组织,并从易碎的胚性细胞中分离原生质体。进一步描述了转化木薯或密切相关物种的原生质体的方法以及由此获得的转化的原生质体。另外,描述了从这些原生质体再生植物的方法,并由此获得了木薯植物或紧密相关的物种。从此类木薯植物的块茎中分离的淀粉的支链淀粉含量为淀粉干重的至少约90%重量。

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