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Enhanced production of Streptococcus mutans mutacin I and III

机译：变形链球菌诱变剂I和III的产量提高

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摘要

Methods for producing a mutacin, by growing a mutacin-producing cell in a liquid medium under conditions in which mutacin is produced and isolating mutacin from the liquid medium. Methods are also provided for isolating and purifying mutacin I/III from fermentation broths of Streptococcus mutans strains CH43 and UA787 to homogeneity. Fermentation are conducted in anaerobic bioreactors under anaerobic conditions at a cultivation temperature of about 35-42° C., with agitation rate of between 50-250 rpm.

机译：通过在产生诱变剂的条件下在液体培养基中生长产生诱变素的细胞并从液体培养基中分离出诱变素的方法来制备诱变素。还提供了从变形链球菌CH43和UA787菌株的发酵液中分离和纯化突变蛋白I / III的方法。在厌氧条件下，在约35-42℃的培养温度下，在厌氧生物反应器中进行发酵。搅拌速度在50-250 rpm之间。

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公开/公告号US2004146983A1

专利类型
公开/公告日2004-07-29

原文格式PDF
申请/专利权人 CAUFIELD PAGE W.;HE ZENGGUO;
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申请/专利号US20030672784
发明设计人 PAGE W. CAUFIELD;ZENGGUO HE;
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申请日2003-09-26
分类号C12P21/02;C12N1/21;
国家 US
入库时间 2022-08-21 23:21:07

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