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HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS

机译：高速，自动，连续流动，多维分子选择和分析

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摘要

The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second "dimension". The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.

机译：本发明提供了新颖的方法，该方法用于筛选样品以选择目标靶标的配体并获得有关该配体及其结合特性的信息。具体地，所要求保护的多维方法涉及将异质配体的溶液与感兴趣的靶标结合以基于一种或多种结合特性筛选配体。具有第一结合特性的配体与目标靶标结合，从而形成靶标/配体复合物。然后任选地使用多种分离技术中的任何一种将复合物与未结合的组分分离，例如尺寸排阻。然后将至少一个复杂或未绑定的组件引入第二个“维度”。第二维能够基于第二结合特性来分离组分。然后洗脱具有所需结合特性的配体。

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公开/公告号EP0835446B1

专利类型
公开/公告日2003-04-09

原文格式PDF
申请/专利权人 PERSEPTIVE BIOSYSTEMS INC.;
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申请/专利号EP19960923455
发明设计人 JINDAL SATISH;REGNIER FRED E.;WILLIAMS KEVIN;AFEYAN NOUBAR B.;PALIWAL SANDEEP;EVANS DAVID;PINGALI ARUNA;
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申请日1996-06-26
分类号G01N30/46;
国家 EP
入库时间 2022-08-21 23:54:02

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