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Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
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机译:使用突变型RNA聚合酶的方法,可减少非规范性和规范性三磷酸核苷之间的区别
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摘要
A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate.
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