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METHOD FOR DETECTING DISEASED PART SUCH AS CANCER OR INFLAMMATION OF TRANSGENIC MOUSE INTEGRATED WITH 3' UNTRANSLATED REGION OF CYTOKINE USING INSTABILITY OF mRNA
METHOD FOR DETECTING DISEASED PART SUCH AS CANCER OR INFLAMMATION OF TRANSGENIC MOUSE INTEGRATED WITH 3' UNTRANSLATED REGION OF CYTOKINE USING INSTABILITY OF mRNA
PROBLEM TO BE SOLVED: To analyze a change before a mutation of a genomic DNA is occurred with a certain and simple method by using a transgenic mouse and to simply identify the diseased part affected with the change. ;SOLUTION: This method comprises producing the transgenic mouse by using a construct in which cDNA such as a green fluorescence protein(GFP) and a -galactosidase alkaline phosphatase which can be a reporter gene is integrated into a gene expression vector having 3' untranslated region (3'-UTR) containing a poly A recognition sequence of an elongation factor-1 (EF-1) promoter and a Granulocyte colony stimulating factor (G-CSF), irradiating the transgenic mouse with a radiation rays or ultraviolet rays to make only cancerous or stressed cells emit a light or develop a color and identifying the diseased part.;COPYRIGHT: (C)2003,JPO
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