首页>
外国专利>
VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA
VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA
FIELD: biotechnology, gene engineering, veterinary science, medicine. SUBSTANCE: due to amplification and cloning the viral genome fragment of chicken variola of a native K strain it is obtained a TK-gene- containing DNA sequence insufficient for viral replication. By using the obtained DNA sequence, due to gene engineering techniques and manipulations pTK1FPV and pTK2FPV recombinant plasmids are obtained which contain 5 and 3-terminal parts of TK-gene. Due to these and other additional plasmids a pINTFPV1 plasmid vector is obtained which is able to integrate into chicken viral genome. This technical solution could be applied at obtaining gene engineering vaccines based upon chicken variola virus. EFFECT: higher efficiency. 4 cl, 5 dwg, 5 ex
展开▼
机译：领域：生物技术，基因工程，兽医学，医学。物质：由于扩增和克隆了天然K株鸡天花的病毒基因组片段，因此获得了含有TK基因的DNA序列，不足以进行病毒复制。通过使用获得的DNA序列，由于基因工程技术和操作，获得了包含TK基因的5和3末端部分的pTK1FPV和pTK2FPV重组质粒。由于这些和其他附加质粒，获得了能够整合到鸡病毒基因组中的pINTFPV1质粒载体。该技术方案可用于获得基于鸡天花病毒的基因工程疫苗。效果：更高的效率。 4 cl，5 dwg，5 ex
展开▼
