首页> 外国专利> METHOD FOR PRODUCTION AND CRYOPRESERVATION OF BOVINE EMBRYOS DEVELOPED IN VITRO AND FROZEN STRAW FOR DIRECT TRANSFERRING SAME

METHOD FOR PRODUCTION AND CRYOPRESERVATION OF BOVINE EMBRYOS DEVELOPED IN VITRO AND FROZEN STRAW FOR DIRECT TRANSFERRING SAME

机译:直接转移的冻存秸秆体外培养和冷冻的牛胚胎的生产和冷冻方法

摘要

The invention concerns a method for producing cryopreserved bovine embryos from bred oocytes in vitro comprising the following steps: 1) maturing and breeding bovine oocytes in vitro; 2) culturing in vitro said bred oocytes until the morula or blastocyst stage; 3) selecting quality 1 embryos; 4) freezing the selected embryos. The culturing step (2) is a co-culture on a mat of Vero cells carried out serum-free, and the freezing step (4) is carried out while the embryo is conditioned in a freezing medium containing a glycerol-trehalose mixture as cryoprotectant.
机译:本发明涉及一种从繁殖的卵母细胞体外制备冷冻保存的牛胚胎的方法,其包括以下步骤:1)在体外成熟和繁殖牛卵母细胞; 2)在体外培养所述繁殖的卵母细胞直到桑ula或胚泡期; 3)选择优质1个胚胎; 4)冷冻选定的胚胎。培养步骤(2)是在无血清的Vero细胞垫上共培养,并且冷冻步骤(4)是在将胚置于含有甘油-海藻糖混合物作为冷冻保护剂的冷冻培养基中调理的同时进行的。

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