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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Fingerprinting Quality Control of Media for In Vitro Bovine Embryo Production Using Direct Infusion Automated Chip-Based Nano-Electrospray Mass Spectrometry
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Fingerprinting Quality Control of Media for In Vitro Bovine Embryo Production Using Direct Infusion Automated Chip-Based Nano-Electrospray Mass Spectrometry

机译：使用直接输注自动芯片的纳米电喷雾质谱法进行体外牛胚胎生产介质的指纹质量控制

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摘要

In a commercial bovine in vitro production (IVP) routine, media are fundamental for satisfactory results. Quality evaluation of media used for commercial bovine embryo production is usually performed by measuring the pH, the osmolarity and the embryo in vitro development rates, which may not evidence sub-optimal media condition. Sub-optimal embryo culture conditions cause economic losses especially due to low embryo viability after transfer to the recipient cow. On human assisted reproduction area, the efficiency of media quality evaluation assays protocols is discussed, and there is a strong recommendation that results from the mouse embryo assay (MEA) be associated to other methods of evaluation. Mass spectrometry fingerprinting has been successfully applied to many products such as for protein digests, beverages, vegetable oils and snake venoms. The aim of this work was to evaluate the potential of ESI mass spectrometry fingerprinting as a high throughput tool for quality control evaluation of media used for bovine in vitro productions.
机译：在商业牛体外生产（IVP）常规中，媒体对令人满意的结果是基础。用于商业牛胚产量的培养基的质量评估通常通过测量pH，渗透性和胚胎体外发育率而进行，这可能不是证据次优媒体状况。次优胚培养条件导致经济损失，特别是由于在转移到受体奶牛后的胚胎活力低。在人类辅助再现面积上，讨论了媒体质量评估测定协议的效率，并且存在强烈推荐，从小鼠胚胎测定（MEA）与其他评价方法相关联。质谱已经成功地应用于许多产品，例如蛋白质消化，饮料，植物油和蛇毒液。这项工作的目的是评估ESI质谱的潜力指纹识别作为用于牛体外生产的培养基的质量控制评估的高通量工具。

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