"INSULATED NUCLEIC ACID FRAGMENT, POLYPEPTID, CHEMERIC GENE, MICROORGANISM, RECOMBINANT MICROORGANISM, RECOMBINANT E. COLI LINE, E. COLI RECOMBINING29 VET PETROPETOUS E32, COLI LINE8 RJ8 LINK 3-PROPANODIOL ". The present invention provides an improved method of biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process of converting glucose to 1,3-propanediol is achieved by using an E. coli transformed with the dha dhaR, orfY, dhaT, orfX, orfW, dhaB1, Klebsiella pneumoniae dhaB2, dhaB3 and orfZ, all of these genes arranged in the same genetic organization found in wild-type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for producing 1,3-propanediol from glucose by using a recombinant E. coli containing G3PDH coding genes, a G3P phosphatase, a dehydratase and a dehydratase reactivation factor compared to a similar process using a recombinant E. coli containing G3PDH coding genes, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The highly improved process depends on the presence in E. coli of a gene encoding non-specific catalytic activity sufficient for the conversion of 3-hydroxypropionaldehyde to 1,3-propanediol.
展开▼
机译：“绝缘的核酸片段，多肽，化学基因，微生物，重组微生物，重组大肠杆菌E. COLI LINE，大肠杆菌E. COLI Recombining29 VET PETPETPETOUS E32，COLI LINE8 RJ8 LINK 3-PRODUPODIOL”。本发明提供了在单个微生物中从可发酵碳源生物生产1，3-丙二醇的改进方法。在本发明的一个方面，通过使用用dha dhaR，orfY，dhaT，orfX，orfW，dhaB1，肺炎克雷伯菌肺炎dhaB2，dhaB3和dhad转化的大肠杆菌来实现将葡萄糖转化为1，3-丙二醇的改进方法。 orfZ，所有这些基因都以在野生型肺炎克雷伯菌中发现的相同遗传组织排列。在本发明的另一方面，与使用α-淀粉酶的类似方法相比，通过使用包含G3PDH编码基因，G3P磷酸酶，脱水酶和脱水酶再活化因子的重组大肠杆菌从葡萄糖生产1，3-丙二醇的改进方法。重组大肠杆菌，其中包含G3PDH编码基因，G3P磷酸酶，脱水酶，脱水酶再活化因子和1,3-丙二醇氧化还原酶（dhaT）。高度改进的方法取决于在大肠杆菌中是否存在编码非特异性催化活性的基因，该基因足以将3-羟基丙醛转化为1,3-丙二醇。
展开▼
