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METHOD FOR TRANSFECTION OF AVIAN PRIMORDIAL GERM CELLS
METHOD FOR TRANSFECTION OF AVIAN PRIMORDIAL GERM CELLS
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机译:航空原代生殖细胞的翻译方法
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摘要
The present invention relates to a method for transfection of avian primordial germ cells which comprises a step of electroporating a foreign gene into avian primordial germ cells after addition of dimethylsulfoxide( DMSO) to culture medium containing the cells, and a method for production of transgenic Aves by employing the avian primordial germ cells transfected thereby. The DMSO-electroporation method of the invention has various advantages over the conventional transfection methods in light of high transfection efficiency, safety, and simple manipulation. Accordingly, a large number of primordial germ cells transfected with a gene of interest can be efficiently produced by the invention, which, in turn, may increase the rate of transfer of exogenous DNA into recipient embryos.
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