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Novel reagents for the determination of the redox phenomenon in protein peptide, a kit for the method and

机译:用于测定蛋白质肽中氧化还原现象的新型试剂,用于该方法的试剂盒和

摘要

(57) [Abstract] is for analyzing the amount of methionine sulfoxide in the protein sample, the first example of this method with methionine sulfoxide reducing agent in the presence of a reducing agent that has a reporter tag covalently linked wherein the step of constructing a protein solution, the reducing agent is oxidized by this. The reducing agent oxidized form, which is intended corresponds to the amount of methionine sulfoxide in the sample, is quantified. A second example of this method is to analyze the amount of disulfide bonds in the protein sample or polypeptide. The present invention proceeds as described above, but sometimes there enzyme that absence. , Discloses the kit with a reducing agent to label fluorescently novel for carrying out the process is given as well.
机译:(57)[摘要]用于分析蛋白质样品中蛋氨酸亚砜的量,该方法的第一个实例是在具有共价连接的报告子标签的还原剂存在下,用蛋氨酸亚砜还原剂进行的,其中在蛋白质溶液中,还原剂由此被氧化。定量还原剂的氧化形式,该形式应与样品中甲硫氨酸亚砜的量相对应。该方法的第二个例子是分析蛋白质样品或多肽中二硫键的数量。如上所述进行本发明,但是有时会缺乏这种酶。 ,还公开了用还原剂标记试剂盒以进行荧光标记的新颖方法。

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