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Novel reagents for the determination of the redox phenomenon in protein peptide, a kit for the method and
Novel reagents for the determination of the redox phenomenon in protein peptide, a kit for the method and
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机译:用于测定蛋白质肽中氧化还原现象的新型试剂,用于该方法的试剂盒和
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摘要
(57) [Abstract] is for analyzing the amount of methionine sulfoxide in the protein sample, the first example of this method with methionine sulfoxide reducing agent in the presence of a reducing agent that has a reporter tag covalently linked wherein the step of constructing a protein solution, the reducing agent is oxidized by this. The reducing agent oxidized form, which is intended corresponds to the amount of methionine sulfoxide in the sample, is quantified. A second example of this method is to analyze the amount of disulfide bonds in the protein sample or polypeptide. The present invention proceeds as described above, but sometimes there enzyme that absence. , Discloses the kit with a reducing agent to label fluorescently novel for carrying out the process is given as well.
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