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Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B
Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B
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机译:B型流感嗜血杆菌外膜蛋白P2的高水平表达,纯化和重折叠的方法
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摘要
The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.
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