首页> 外国专利> Dna fragment gc3 subject to neisseria gonorrhoeae neisseria meningitidis non-species-related breakpoints *

Dna fragment gc3 subject to neisseria gonorrhoeae neisseria meningitidis non-species-related breakpoints *

机译:DNA片段gc3受到淋病奈瑟菌脑膜炎奈瑟菌非物种相关断点的影响*

摘要

Disclosed herein is a newly-identified DNA sequence from Neisseria gonorrhoeae designated GC3. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the species-specific detection of N. gonorrhoeae. Preferably, N. gonorrhoeae is detected by amplifying the N. gonorrhoeae nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, N. gonorrhoeae nucleic acids are amplified and detected by thermophilic strand displacement amplification (tSDA). Also preferred are methods for detecting N. gonorrhoeae that are employed in conjunction with methods for detecting other sexually-transmitted pathogens, in particular, Chlamydia trachomatis.
机译:本文公开了来自淋病奈瑟氏球菌的新鉴定的DNA序列,称为GC3。还公开了用于淋病奈瑟菌的物种特异性检测的方法,寡核苷酸探针,扩增引物和试剂盒。优选地,通过使用公开的扩增引物扩增淋病奈瑟氏球菌核酸,然后检测扩增的核酸来检测淋病奈瑟氏球菌。在一个更优选的实施方案中,通过嗜热链置换扩增(tSDA)扩增和检测淋病奈瑟氏球菌核酸。还优选的是用于检测淋病奈瑟氏球菌的方法,其与用于检测其他性传播病原体,特别是沙眼衣原体的方法结合使用。

著录项

  • 公开/公告号FR2778415B1

    专利类型

  • 公开/公告日2001-04-06

    原文格式PDF

  • 申请/专利权人 BECTON DICKINSON AND COMPANY;

    申请/专利号FR19990005488

  • 发明设计人 YOU QIMIN;

    申请日1999-04-27

  • 分类号C12Q1/68;C07H21/04;G01N33/569;

  • 国家 FR

  • 入库时间 2022-08-22 01:08:03

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