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Dna fragment gc3 subject to neisseria gonorrhoeae neisseria meningitidis non-species-related breakpoints *
Dna fragment gc3 subject to neisseria gonorrhoeae neisseria meningitidis non-species-related breakpoints *
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机译:DNA片段gc3受到淋病奈瑟菌脑膜炎奈瑟菌非物种相关断点的影响*
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摘要
Disclosed herein is a newly-identified DNA sequence from Neisseria gonorrhoeae designated GC3. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the species-specific detection of N. gonorrhoeae. Preferably, N. gonorrhoeae is detected by amplifying the N. gonorrhoeae nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, N. gonorrhoeae nucleic acids are amplified and detected by thermophilic strand displacement amplification (tSDA). Also preferred are methods for detecting N. gonorrhoeae that are employed in conjunction with methods for detecting other sexually-transmitted pathogens, in particular, Chlamydia trachomatis.
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