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Detecting Meticillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci comprises using a flow-through cytometer and fluorescence readings
Detecting Meticillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci comprises using a flow-through cytometer and fluorescence readings
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机译：检测耐甲氧西林的金黄色葡萄球菌和耐万古霉素的肠球菌包括使用流式细胞仪和荧光读数
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摘要
Detecting Meticillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) in a flow-through cytometer is new. Detecting Meticillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) in a flow-through cytometer, comprises: (a) preparing a bacterial suspension; (b) growing of the bacteria in nutrient solution; (c) adding at least one fluorescent-labelled indicator, specific for one of the bacterial species; (d) adding a fluorescent-labelled indicator for damaged cells and an antibiotic to test the resistance; (e) running the suspension through the flow cytometer and radiating each bacteria with a laser; (f) measuring the fluorescence of each bacteria as the laser hits it; and (g) evaluating the total of the single measurements to determine the resistance of the bacteria to the tested antibiotic.
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