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Separation and Purification Method of the Isoflavon as Bioactive Material from the Soybean Embryo
Separation and Purification Method of the Isoflavon as Bioactive Material from the Soybean Embryo
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机译：大豆胚中异黄酮作为生物活性物质的分离纯化方法
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摘要
PURPOSE: Methods for purifying and isolating isoflavone in a high yield are provided. Thereby, isoflavone, which is a physiologically active ingredient, is isolated and purified from the embryo of soy bean obtained when raw soy bean is processed. CONSTITUTION: A method for purifying and isolating isoflavone, which is a physiologically active ingredient from the embryo of soy bean is composed of the following steps: (a) removing fat from the embryo of soy bean; (b) extracting it with alkaline aqueous solution, to remove fiber and protein; (c) flowing the resultant extracted solution onto absorption resin column, to absorb isoflavone and saponin and then isolating these materials with a polar solution (d) drying them under reduced pressure in infrared ray furnace, to obtain a mixture of isoflavone and saponin; and (e) purification and isolation of isoflavone from the mixture using acetone.
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