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Surface modification of microtiter plates, useful in chemical assays, immunoassays or drug screening assays, comprises forming insoluble polymer film

机译：用于化学分析，免疫分析或药物筛选分析的微量滴定板的表面修饰包括形成不溶性聚合物膜

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摘要

Method for modifying the surface of microtiter plates comprises chemical or photochemical grafting, radical or ionic polymerization or polymer crosslinking, including molecular impact polymerization, to form a stable insoluble film that can be used to monitor the formation and/or conversion of substances in solution and/or on the surface of the microtiter plate. Independent claims are also included for the following: (1) a method for determining the pH of samples by contacting them with the modified microtiter plates and measuring the light absorption of the polymer film; (2) a method for determining substances in contact with the modified microtiter plates, in which one or more enzymes, receptors, antibodies or cells are immobilized on the polymer surface, comprising measuring the change in the optical properties of the polymer film caused by a protonation/deprotonation or redox redox reaction in the course of the binding and/or catalytic conversion of the substances; (3) an enzyme-linked immunosorbent assay (ELISA) method in which the antibodies, receptors or antigens immobilized on the microtiter plate surface are replaced by molecular impact polymers (MIPs); (4) a drug screening method in which the receptors or ligands immobilized on the microtiter plate surface are replaced by MIPs; (5) an ELISA method in which antibodies, receptors or antigens are immobilized on the surface of the modified microtiter plates; (6) an assay based on the modified microtiter plates in which a change in absorption spectrum (wavelength), radioactivity, fluorescence, phosphorescence, chemiluminescence or bioluminescence is used for quantitative determination; (7) a method for monitoring cell cultures, comprising measuring pH, substrate concentration or metabolite concentration with the modified microtiter plates; (8) a method for surface modification of optical elements (fibers or films) by chemical or photochemical grafting, radical or ionic polymerization or polymer crosslinking, including molecular impact polymerization, to form a stable insoluble film that can be used to monitor the formation and/or conversion of substances in solution and/or on the surface of the optical element; and (9) use of the polymer-modified optical elements of (8) in sensors.

机译：修饰微量滴定板表面的方法包括化学或光化学接枝，自由基或离子聚合或包括分子碰撞聚合在内的聚合物交联，以形成稳定的不溶膜，该膜可用于监测溶液中物质的形成和/或转化。 /或在微量滴定板的表面上。还包括以下方面的独立权利要求：（1）通过使样品与改性的微量滴定板接触并测量聚合物膜的光吸收来确定样品的pH的方法; （2）一种确定与修饰的微量滴定板接触的物质的方法，其中将一种或多种酶，受体，抗体或细胞固定在聚合物表面上，该方法包括测量由聚合物引起的聚合物膜的光学性能变化。在物质结合和/或催化转化过程中的质子化/去质子化或氧化还原-氧化还原反应; （3）酶联免疫吸附法（ELISA），其中固定在微量滴定板表面上的抗体，受体或抗原被分子撞击聚合物（MIP）代替; （4）一种药物筛选方法，其中固定在微量滴定板表面上的受体或配体被MIP代替; （5）一种ELISA方法，其中将抗体，受体或抗原固定在修饰的微量滴定板的表面上; （6）基于改进的微量滴定板的测定，其中吸收光谱（波长），放射性，荧光，磷光，化学发光或生物发光的变化用于定量测定; （7）一种监测细胞培养物的方法，包括用改良的微量滴定板测量pH，底物浓度或代谢产物浓度。（8）一种通过化学或光化学接枝，自由基或离子聚合或包括分子碰撞聚合在内的聚合物交联来对光学元件（纤维或薄膜）进行表面改性的方法，以形成稳定的不溶性膜，该膜可用于监测形成和形成。 /或溶液中和/或光学元件表面上物质的转化; （9）在传感器中使用（8）的聚合物改性的光学元件。

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公开/公告号DE19832598A1

专利类型
公开/公告日2000-03-09

原文格式PDF
申请/专利权人 POLY-AN GMBH;
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申请/专利号DE1998132598
发明设计人 SERGEYEVA TETYANA;PILETSKY SERGIY;PILETSKA OLENA;SCHEDLER UWE;PANASYUK TETYANA;GANNA ELSKA;ULBRICHT MATHIAS;
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申请日1998-07-09
分类号G01N33/543;B01L3/00;
国家 DE
入库时间 2022-08-22 01:42:40

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