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Quantification of blood cell activity with ELISPOT-assay and a microtiter plate
Quantification of blood cell activity with ELISPOT-assay and a microtiter plate
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机译:用ELISPOT测定和微量滴定板定量血细胞活性
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摘要
Determining the activity of human or animal cells, especially blood cells, comprises incubating the cells in the presence of cell reaction inducing foreign substances, using and ELISA spot method. The reactive cells release a substance, which results in a color reaction on the specific spots. The color reaction is used as a measure of the cell reactivity. Independent claims are also included for: (1) a microtitre plate, especially a 96 hole plate, where the floors of the holes have an area of less than 15 (especially less than 10) mm2; and (2) a kit comprising (1) and all the relevant agents, such as capture antibodies and antigens, and color reagents.
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