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A process for producing beta-galactosidase recombinant Thermus sp. (Strain t2) in host cells, and purifying in a single chromatographic step.
A process for producing beta-galactosidase recombinant Thermus sp. (Strain t2) in host cells, and purifying in a single chromatographic step.
A process for producing recombinant {b galactosidase Themus SP. (Cepa T2) in host cells, and purifying in a single chromatographic step. Unpublished sequence nucleotides corresponding to a gene of Thermus sp. (Cepa T2: ATCC 27737) and encoding a thermostable boot-galactosidase. Obtaining genetic expression for high said nucleotide sequence in cells Eacherichia coli by its cloning expression vectors constructs. Purification of a hybrid protein with beta-galactosidase activity produced thermostable in E. coli in a single chromatographic step. This chimeric protein thermostable has application in hydrolysis of milk and milk products, reducing the lactose content in effluent sera dairy, cold labeling nucleic acid probes by conjugation therewith, synthesis of oligosaccharides by transglycosylation and rapid evaluation of treatments moderate heat.
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