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Retroviral vector, a replication system for said vector and avian or mammalian cells transfected with said vector

机译:逆转录病毒载体,所述载体和用所述载体转染的禽或哺乳动物细胞的复制系统

摘要

A retroviral vector comprising a retrovirus with infectivity for birds and/or mammals in which at least part of the genomic RNA sequences carrying information for the production of viral proteins required in trans for retroviral replication have been replaced by one or more sequences carrying information to be introduced in a target cell chromosome, characterized in that the primer binding site (PBS) has been modified to a sequence that does not allow strong base pairing with the 3' end or other priming sequence in any naturally occurring tRNA. The present application is directed to the tRNA-like primer.
机译:包含对鸟类和/或哺乳动物具有传染性的逆转录病毒的逆转录病毒载体,其中至少一部分携带有用于产生逆转录病毒复制所需的反式病毒所需病毒蛋白的信息的基因组RNA序列已被一个或多个携带有待复制信息的序列取代。插入靶细胞染色体中的RNA,其特征在于引物结合位点(PBS)已被修饰为不允许与任何天然存在的tRNA中的3'端或其他引物序列强碱基配对的序列。本申请针对tRNA样引物。

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