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Quadruplex PCR systems consisting of STR loci which are high discriminating in Korean population and the methods of human identification using them
Quadruplex PCR systems consisting of STR loci which are high discriminating in Korean population and the methods of human identification using them
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机译：在韩国人群中具有高度区分性的由STR基因座组成的四链PCR系统及其鉴定方法
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摘要
The invention can quickly gene forensics by amplifying the combination of STR loci using the polymerase chain reaction (PCR) relates to a gene forensics method using the detection and combinations of genetic markers with the discrimination in the Korean population in the genetic system, while It will be on the way in. Using gene forensics method using a multiplex PCR system of the present invention, at least three or more, which may be amplified at the same time, and preferably obtaining at least one analysis of a DNA sample that has four STR loci, specific primers in the DNA sample. and characterized in that it comprises a step of evaluating the step of detecting the amplified products by electrophoresis amplified alleles to PCR amplify the STR sequence, and this determines the respective genotypes of the analyzed locus combinations in the DNA sample through It shall be. In addition, multiplex STR system, consisting of the at least three or more loci completed by the present inventors are D5S818, D13S317, D19S253, D3S2406, D2S1371, D8S1477, D12S391, D20S470, D6S1043, D9S925, D7S821, D4S2368, D21S1437, D16S3253, from the group consisting of D14S608 and D18S1270 loci characterized by consisting of the combination of the selected at least three or more loci.
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