首页> 外国专利> BANANA PROTEINS, DNA, AND DNA REGULATORY ELEMENTS ASSOCIATED WITH FRUIT DEVELOPMENT

BANANA PROTEINS, DNA, AND DNA REGULATORY ELEMENTS ASSOCIATED WITH FRUIT DEVELOPMENT

机译:与水果发育相关的香蕉蛋白,DNA和DNA调控元素

摘要

The present invention provides isolated and purified genes which aredifferentially expressed during banana fruit development, and the proteinproducts of these genes. The present invention further provides DNA regulatoryelements which are differentially expressed during banana fruit development,chimeric genes comprising these DNA regulatory elements operably linked toheterologous DNA molecules, and plants transformed with said chimeric genes,providing for controlled expression of said heterologous DNA molecules duringthe development and ripening of the fruit of said plants, or in response toexogenous ethylene signals in said plants. The present invention also providesa method for expression of a heterologous protein in fruit comprisingtransforming fruiting plants with one or more chimeric genes according to thepresent invention, exposing said fruit to an endogenous or exogenous ethylenesignal, and harvesting fruit containing said heterologous protein. The methodof the present invention may further comprise isolating the proteins producedby said method from the harvested fruit. In a particularly preferredembodiment, the heterologous proteinis a therapeutic protein, which may beisolated from the harvested fruit, or consumed directly in the transformedfruit by a patient in need of said therapeutic protein.
机译:本发明提供了分离和纯化的基因,它们是香蕉果实发育过程中差异表达的蛋白质这些基因的产物。本发明进一步提供了DNA调节在香蕉果实发育过程中差异表达的元素包含与这些DNA调控元件有效连接的嵌合基因异源DNA分子,以及用上述嵌合基因转化的植物,提供在所述过程中所述异源DNA分子的受控表达所述植物果实的发育和成熟,或响应所述植物中的外源乙烯信号。本发明还提供在水果中表达异源蛋白质的方法,包括根据一个或多个嵌合基因转化具有结果的植物本发明,将所述水果暴露于内源或外源乙烯信号,并收获含有所述异源蛋白质的果实。方法本发明的方法还可包括分离产生的蛋白质通过上述方法从收获的果实中提取。在一个特别优选的在一个实施方案中,异源蛋白是治疗蛋白,其可以是从收获的果实中分离出来,或直接在转化后的果实中食用需要所述治疗性蛋白质的患者的水果。

著录项

  • 公开/公告号CA2304109A1

    专利类型

  • 公开/公告日1999-04-01

    原文格式PDF

  • 申请/专利号CA19982304109

  • 发明设计人 MAY GREGORY;CLENDENNEN STEPHANIE;

    申请日1998-09-23

  • 分类号C12N15/29;A01H5/00;C07K14/415;C07K14/42;C07K14/825;C12N5/10;C12N9/08;C12N9/10;C12N9/24;C12N15/53;C12N15/54;C12N15/55;C12N15/82;

  • 国家 CA

  • 入库时间 2022-08-22 02:23:40

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