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Exogenous gene expression vector containing chick -actin gene promoter

机译:含有鸡肌动蛋白基因启动子的外源基因表达载体

摘要

An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick -actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.
机译:一种用于在动物细胞中表达外源基因的表达载体,其包含鸡肌动蛋白基因启动子和用于在所述启动子的下游掺入外源基因的限制性酶切位点以及表达外源基因的方法,其包括在限制性内切酶位点处将所述外源基因掺入表达载体中以掺入外源基因,将所述载体引入动物细胞中并培养获得的转化动物细胞。本发明的表达系统可以应用于任何外源基因的表达,并且具有极高的表达效率,并且适用于广泛的宿主细胞,因此可以充分用于工业规模生产有用的材料。 。

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