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Use of the lectin Sambucus nigra to quantify the terminal sialic acid residues of the human transferrin molecule using a fully immunoenzymatic method (EIA)
Use of the lectin Sambucus nigra to quantify the terminal sialic acid residues of the human transferrin molecule using a fully immunoenzymatic method (EIA)
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机译：使用完全免疫酶法（EIA）使用凝集素黑接骨吸血鬼来量化人转铁蛋白分子的末端唾液酸残基
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摘要
The subject matter of this invention is a method of determining terminal sialic acid residues in human transferrin according to a sandwich principle, which is characterized in that the sample fluid containing the human transferrin is incubated with a first receptor which binds specifically to human transferrin, the thus-formed complex is separated from the sample fluid and incubated with a second receptor which binds specifically to terminal sialic acid residues in human transferrin, the second receptor being bound or able to bind to a marker, and the complex made up of the first receptor, human transferrin and the second receptor is determined with the marker. According to one embodiment, the method of the invention allows the determination of sialic-acid-deficient human transferrin in body fluids.
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