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Use of the lectin Sambucus nigra to quantify the terminal sialic acid residues of the human transferrin molecule using a fully immunoenzymatic method (EIA)
Use of the lectin Sambucus nigra to quantify the terminal sialic acid residues of the human transferrin molecule using a fully immunoenzymatic method (EIA)
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机译：使用完全免疫酶法（EIA）使用凝集素黑接骨吸血鬼来量化人转铁蛋白分子的末端唾液酸残基
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摘要
The object of the present invention is a process for detecting end-position sialic acid groups of human transferrin using the sandwich principle. The process is characterised in that the sample fluid containing the human transferrin is incubated with a first receptor capable of bonding specifically with human transferrin, the complex thus formed is separated from the sample fluid, incubated with a second receptor capable of binding specifically to end-position sialic acid groups of human transferrin, the second receptor being bound or capable of binding to a marker, and the complex of first receptor, human transferrin and second receptor is determined by the marker. In one embodiment, the claimed process facilitates determination of sialic acid-deficient human transferrin in body fluids.
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