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Poly antigen and recombinant things for detecting Cytomegalovirus-specific IgM in human sera by enzyme immunoassay
Poly antigen and recombinant things for detecting Cytomegalovirus-specific IgM in human sera by enzyme immunoassay
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机译：酶免疫法检测人血清中巨细胞病毒特异性IgM的多抗原和重组物
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摘要
And poly - - recombinant mono can be completely replaced to viral antigens (57) when it is used in the [summary] enzyme immunoassay mixture of epitope protein material is disclosed. Poly this mixture, - a epitope fusion protein, this mixture, first region having a (H10), COOH of viral protein pp150 amino acid sequence corresponding to 233 amino acid end of the part or COOH end of the viral protein pp52 It corresponds to the sequence derived from between aa614 and aa595 traveling in direction 5 '→ 3' of pp150 same viral proteins and regions, having a second (F3) the amino acid sequence corresponding to the 43 amino acids at the end of its edges or between aa510 and aa297 to proceed in the direction 5 '→ 3' of pp65 major matrix protein that further fusion proteins of the first comprising a third region having a (A1C2), is encoded by the UL83 viral gene amino acid sequence obtained from between aa373 and aa117 to proceed in the direction 5 '→ 3' of pp38 viral assembly protein encoded by the viral gene UL80a and fusion protein, the second comprising the sequence of amino acids corresponding to the sequence from between and having associated therewith a fusion protein of the third comprising the sequence of amino acids corresponding to the sequence. Fusion proteins of these three can be combined together for the preparation of ELISA kits cytomegalovirus specific IgM detection in human serum.
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