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Method of monitoring endospore-forming bacteria
Method of monitoring endospore-forming bacteria
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机译:监测内生孢子形成细菌的方法
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摘要
Endospore-forming bacteria present in a sample are detected or identified by adding to the sample primers which are specific for conserved regions in a coding region in the genome of the bacteria, and carrying out a DNA amplification procedure. In the detection method, any of the conserved regions present in the sample may be amplified, followed by analysing for the presence of the conserved regions. In the identification method, the conserved regions of the coding region are amplified, followed by analysing the amplified DNA obtained, the primers being such that they simultaneously amplify a variable coding sequence of DNA intermediate the conserved regions of the coding region, the length of the DNA fragment obtained from the amplification step being characteristic of a particular endospore-forming bacterium.
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