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ACCUMULATION OF HEAT SHOCK PROTEINS FOR EVALUATING BIOLOGICAL DAMAGE DUE TO CHRONIC EXPOSURE OF AN ORGANISM TO SUBLETHAL LEVELS OF POLLUTANTS
ACCUMULATION OF HEAT SHOCK PROTEINS FOR EVALUATING BIOLOGICAL DAMAGE DUE TO CHRONIC EXPOSURE OF AN ORGANISM TO SUBLETHAL LEVELS OF POLLUTANTS
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机译:积累热休克蛋白以评估生物体长期暴露于污染物的潜在水平所造成的生物危害
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摘要
Method of detecting chronic exposure of an organism to a pollutant, and forevaluating biological damage due to chronic exposure to sublethal levels ofpollutants and kits for carrying out the method are disclosed. The methodscomprise:(a) sampling at least one organism in order to determinewhether it has been chronically exposed to a sublethal concentration of one ormore pollutants in its environment, under sampling conditions that do not induceany additional heat shock protein (hsp) response in the organism;(b) obtaining a sample of cells or secretions of said organism,suspected of having elevated levels of heat shock proteins and solubilizing theheat shock proteins in the sample; and(c) measuring the concentration of a heat shock proteinselected from hsp 70, hsp 60 and ubiquitin, in said sample.The invention also provides methods for evaluating biological damage due tochronic exposure to a sublethal concentration of one or more pollutantscomprising detecting chronic exposure of the organism by the above method andthen comparing the measured concentration of hsp to a predetermined standardcalibration curve which correlates hsp concentration with physiologicalimpairment of growth or reproductive processes, and kits for carrying out themethods.
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