首页> 外国专利> Process for the production of melanin in cells of the strain DH5a commercial escherichia coli expression from the same structural gene of tyrosinase enzyme.

Process for the production of melanin in cells of the strain DH5a commercial escherichia coli expression from the same structural gene of tyrosinase enzyme.

机译:从酪氨酸酶的相同结构基因在DH5a商业大肠杆菌表达菌株的细胞中产生黑色素的方法。

摘要

Process for the production of melanin cells TRADE CEPA DH5a Escherichia coli from expression therein OF STRUCTURAL GENE tyrosinase enzyme has built a recombinant plasmid (PME100) carrying the structural gene ENZYME tyrosinase Rhizobium meliloti strain GR4. This plasmid is capable of inducing melanin production in E. COLI AS A RESULT OF EXPRESSION PROMOTER FROM THIS lacZ gene IN CONSTRUCTION AND CONTRIBUTED BY pUC18. GENE EXPRESSION IS CONSTITUTIVE PROMOTER FROM SUCH WITHOUT PRIOR INDUCTION. By the process of the invention is achieved in production, simply, SUFFICIENT AMOUNTS OF melanin pigments INDUSTRIAL APPLICABILITY DADA unquestionably the huge variety of properties possessed.
机译:从其中表达结构基因酪氨酸酶的黑色素细胞产生TRADE CEPA DH5a大肠杆菌的方法已经构建了携带结构基因ENZYME酪氨酸酶melhiti菌株GR4的重组质粒(PME100)。该质粒能够在大肠杆菌中诱导黑色素的产生,这是由于该lacZ基因在构建中表达启动子的结果,并由pUC18贡献。基因表达在没有事先诱导的情况下是组成性的促进因素。通过本发明的方法,可以在生产中简单地获得足够量的黑色素颜料。工业应用性DADA无疑具有多种性能。

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