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Flow cytometry sheath reagent for elimination of red cells with preservation of nucleated cells

机译:流式细胞仪护套试剂,用于消除红细胞并保留有核细胞

摘要

A reagent is described which when mixed with whole blood, lyses the red cells substantially instantly, but does not substantially affect the optical, light scattering properties of the white blood cells. This reagent enables the performance of a five part leukocyte differential by flow cytometry and light scattering in a single sample path white cell analyzer. In its preferred embodiment, the lytic agent combines the functions of leukoprotective and antimicrobial agents, selected nonionic surfactants, a lytic and wetting agent and an appropriate buffer to provide pH buffering and to increase the solution's conductivity and red blood cell lysing capability.
机译:描述了一种试剂,当与全血混合时,该试剂基本上立即裂解红细胞,但基本上不影响白细胞的光学,光散射特性。该试剂可通过流式细胞术和光散射在单个样品路径白细胞分析仪中实现五部分白细胞鉴别。在其优选的实施方案中,溶解剂结合了白细胞保护和抗微生物剂,选择的非离子表面活性剂,溶解和湿润剂以及合适的缓冲剂的功能,以提供pH缓冲作用并增加溶液的电导率和红细胞溶解能力。

著录项

  • 公开/公告号EP0582736A1

    专利类型

  • 公开/公告日1994-02-16

    原文格式PDF

  • 申请/专利权人 ABBOTT LABORATORIES;

    申请/专利号EP19920113700

  • 发明设计人 WONG SHOW-CHU;

    申请日1992-08-11

  • 分类号G01N33/50;

  • 国家 EP

  • 入库时间 2022-08-22 04:39:19

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