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Procedure for the Conservation of Membrane Enzymes preferentially cytochrome oxidase, soluble or reconstructed by lyophilization
Procedure for the Conservation of Membrane Enzymes preferentially cytochrome oxidase, soluble or reconstructed by lyophilization
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机译：膜酶的保存程序，优选细胞色素氧化酶，可溶解或通过冻干重建
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摘要
The present Invention refers to method for Conservation of cytochrome oxidase Enzyme Membranes preferably soluble or reconstituu00eddas by lyophilization.Characterized in that it comprises the following steps: resuspend conventionally isolated cytochrome oxidase to a Protein concentration of 10 to 40 mg / mlIn a Solution containing 50 mm and a KH PO4 Compound detergent to 0.5 percent by adjusting the PH2 7.4; Mix the product to lyophilize at a ratio of 1: 4; freeze-drying Product during a time of 4 hours.Obtaining a soluble cytochrome oxidase; characterized in that comprises contacting the cytochrome oxidase with Liposomes at a temperature of 25 to 35 C.To obtain a proteoliposomes; mixing the obtained product in a 1:1 ratio with the environment to lyophilize and immediately Lyophilized Product during a time of 4 hours.
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