DETECTING METHOD OF FOOD-POISONING BACTERIA AND SENSOR USING THE SAME

• 1. 通过全面检测和定量食物中毒细菌的热稳定直接溶血素相关的基因 ( t d h ) 相关溶血素基因)对产溶血素细菌进行检测和定量的方法 [P] . 中国专利： CN1606622A . 2005-04-13
• 2. 检测农业食品的食源性细菌的搭接芯片的高速实时PCR装置及利用其的食物中毒检测方法 [P] . 中国专利： CN105849283B . 2020.02.14
• 3. Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria [P] . 美国专利： US9150931B2 . 2015-10-06

  机译： 食物中毒细菌检测载体以及食物中毒细菌的检测方法

• 4. FOOD-POISONING BACTERIA DETECTION CARRIER, AND METHOD FOR DETECTING FOOD-POISONING BACTERIA [P] . 欧洲知识产权局专利： EP2570489A1 . 2013-03-20

  机译： 食品中毒细菌检测载体和食品中毒细菌检测方法

• 5. FOOD-POISONING BACTERIA DETECTION CARRIER, AND METHOD FOR DETECTING FOOD-POISONING BACTERIA [P] . 美国专利： US2013143759A1 . 2013-06-06

  机译： 食品中毒细菌检测载体和食品中毒细菌检测方法

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• 1. A Secure Routing Method for Detecting False Reports and Wormhole Attacks in Wireless Sensor Networks [J] . Hyeon Myeong Choi ,Su Man Nam ,Tae Ho Cho . 无线传感网络（英文） . 2013,第3期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Plasma effects on the bacteria Escherichia coli via two evaluation methods [J] . Danijela VUJO?EVI? ,Uro? CVELBAR ,Ur?ka REPNIK . 等离子体科学与技术：英文版 . 2017,第7期
• 4. 使用中应变式称重传感器故障检测方法及步骤 [C] . 姚玉明 . 第七届称重技术研讨会 . 2008
• 5. 细菌性食物中毒检测方法比较分析 [A] . 王奕 . 2016