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USE OF BACTERIAL LUCIFERASE STRUCTURAL GENES FOR CLONING AND MONITORING GENE EXPRESSION IN MICROORGANISMS AND FOR TAGGING AND IDENTIFICATION OF GENETICALLY ENGINEERED ORGANISMS
USE OF BACTERIAL LUCIFERASE STRUCTURAL GENES FOR CLONING AND MONITORING GENE EXPRESSION IN MICROORGANISMS AND FOR TAGGING AND IDENTIFICATION OF GENETICALLY ENGINEERED ORGANISMS
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机译：细菌葡聚糖酶结构基因在微生物中克隆和监测基因表达以及遗传工程化微生物的标记和鉴定中的应用
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摘要
a host microorganism is genetically modified to stably by insertion in one or the other of the chromosomal locations of the major foreign dna fragment comprising a non homologous fragment containing gene lux ab of a bioluminescent bacterium is selected. as v.harveyi, in such a way that the non homologous fragment contained in a stable manner as part of the chromosome of the host microorganism cells as they divide in a growth environment, the genetic expression of microorganisms into the pr production of luciferase.which in turn emits light in the presence of an aldehyde, serving as a measure of gene expression. gram positive bacteria and gram negative bacteria are microorganisms that can be modified in the manner described above.a radiofilm can be used to measure the amount of light emitted from a micro organism with the use of multiple droplets containing the microorganism and having a number of cells (or plasmid) and appropriate.
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