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BACILLUS-S-P, DNA FRAGMENT CONTAINING L-LACTIC ACID DEHYDROGENASE GENE, GENE RECOMBINANT PLASMID CONTAINING THE SAME GENE, L-LACTIC ACID DEHYDROGENASE GENE AND GENE RECOMBINANT PLASMID CONTAINING THE SAME GENE
BACILLUS-S-P, DNA FRAGMENT CONTAINING L-LACTIC ACID DEHYDROGENASE GENE, GENE RECOMBINANT PLASMID CONTAINING THE SAME GENE, L-LACTIC ACID DEHYDROGENASE GENE AND GENE RECOMBINANT PLASMID CONTAINING THE SAME GENE
PURPOSE:To provide a novel thermophile, Bacillus.S.P, which can grow at relatively high temperature, does not cause any gelatin liquefaction, does not hydrolyze starch and produces L-lactic acid dehydrogenase. CONSTITUTION:A thermophile, Bacillus.S.P TP626 (FERM 10450), which can grow at a relatively high temperature such as 50-70 deg.C, does not cause any gelatin liquifaction, does not hydrolyze starch and produces L-lactic acid dehydrogenase was found out as a result of an examination for finding out a L-lactic acid dehydrogenase originated from a thermophile. The isolation of a complex plasmid pTCl containing a L-lactic acid dehydrogenase gene originated from the bacterium stain and a genetic recombination using the gene permits to massively produce the L-lactic acid dehydrogenase.
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