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PROTEIN, IMMOBILIZED ON CARRIER BY PHYSICAL ADSORPTION METHOD AND CAPABLE OF EXHIBITING AEQUORIN ACTIVITY
PROTEIN, IMMOBILIZED ON CARRIER BY PHYSICAL ADSORPTION METHOD AND CAPABLE OF EXHIBITING AEQUORIN ACTIVITY
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机译：通过物理吸附方法固定在载体上的蛋白质，并能显示出秋红素活性
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摘要
PURPOSE:To obtain the subject immobilized protein, readily recoverable and reutilizable after use and useful as biosensor, etc., by physically binding a luminous protein having aequorin activity to an organic or inorganic carrier having the ability to bind to the protein. CONSTITUTION:An organic or inorganic carrier (e.g. activated alumina) having the ability to bind to a protein (e.g. apoaequorin) is swelled in a buffer solution and the swollen carrier is then suspended in a solution containing the protein having aequorin activity and allowed to stand at 37 deg.C for 30min to adsorb the protein thereon. The buffer solution containing the carrier is further subjected to centrifugation and separated into the carrier and a supernatant liquid and the carrier is subsequently washed with the buffer solution to afford the objective immobilized protein having the aequorin activity. Furthermore, a protein having the aequorin activity prepared according to a technique of recombinant DNA is preferably used as the protein having the aequorin activity.
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