首页> 外国专利> METHOD OF DIAGNOSING GIARDIA LAMBLIA SPECIFIC ANTIGEN IN FACES, SINGLE SPECIFIC ANTIBODY FOR THE ANTIGEN AND GIARDIASIS

METHOD OF DIAGNOSING GIARDIA LAMBLIA SPECIFIC ANTIGEN IN FACES, SINGLE SPECIFIC ANTIBODY FOR THE ANTIGEN AND GIARDIASIS

机译：诊断面部贾第鞭毛虫特定抗原的方法，单一抗原和贾第鞭毛虫特异性抗体

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摘要

PURPOSE: To improve the detecting sensitivity for girdia lamblia(G.L.) in feces so as to diagnose the G.L. stably and surely by preparing a G.L.-specific antigen (GSA65) useful for diagnosing G.L. in feces and a single specific antibody to the antigen. CONSTITUTION: At the time of preparing a refined GSA65, a partially refined mixture containing a GSA65 derived from the eluate of feces, the cyst of G.L. or its nutrient, and a contaminant is bonded to a matrix in which a monospecific polyclonal antibody is bonded to the GSA65 and recovered. The GSA65 has a molecular weight of about 65,000 and has a resistance to the resolution and digestion of proteins. After impurities are washed out from the matrix and the matrix is dried for about 30 to 60 minutes, a stable GSA65 Girdia Lamblia cyst precipiti is obtained by partially denaturing the complex by washing the matrix with an acidic water-alcohol mixture and a serum to GSA65 is obtained by introducing the precipiti to a manmmalian animal. When this serum is brought into contact with the eluate of feces, the generation of the complex can be detected under the presence of the G.L.

机译：目的：通过制备可用于诊断粪便中G.L.的G.L.特异性抗原（GSA65）和该抗原的单一特异性抗体，提高粪便中对Girdia lamblia（G.L.）的检测灵敏度，从而稳定，可靠地诊断G.L ..组成：在制备精制的GSA65时，部分精制的混合物包含衍生自粪便洗脱液的GSA65，GL的囊肿或其营养成分以及污染物结合到基质上，在基质上结合了单特异性多克隆抗体GSA65并恢复。 GSA65的分子量约为65,000，对蛋白质的分解和消化具有抵抗力。从基质中洗去杂质并将基质干燥约30至60分钟后，通过用酸性水-醇混合物和血清将基质洗涤至GSA65，使复合物部分变性，从而获得稳定的GSA65 Girdia Lamblia囊肿沉淀物通过将沉淀物引入哺乳动物动物而获得。当该血清与粪便洗脱液接触时，可以在G.L存在下检测复合物的产生。

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公开/公告号JPH03199968A

专利类型
公开/公告日1991-08-30

原文格式PDF
申请/专利权人 AREKUSON BAIOMEDEIKARU INC;
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申请/专利号JP19900087329
发明设计人 JIYON DEI ROZOFU;
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申请日1990-03-30
分类号C07K1/22;C07K14/435;C07K14/44;C07K16/00;C07K16/20;C12P21/00;C12R1/90;G01N33/569;
国家 JP
入库时间 2022-08-22 06:04:55

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