首页> 外国专利> MONOCLONAL ANTIBODY REACTIVE TO SENILE SPOT, CELL STRAIN PRODUCING SAME AND PRODUCTION OF SAID MONOCLONAL ANTIBODY

MONOCLONAL ANTIBODY REACTIVE TO SENILE SPOT, CELL STRAIN PRODUCING SAME AND PRODUCTION OF SAID MONOCLONAL ANTIBODY

机译：单克隆抗体对老年斑，细胞株产生相同的反应和所述单克隆抗体的产生

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摘要

NEW MATERIAL:A monoclonal antibody reactive to senile spot in cerebral structure having 160,000-180,000mol.wt. as a monomer and 800,000-1,000,000mol. wt. as pentamer by polyacrylamide gel secondary electrophoresis using sodium dodecyl sulfate as a protein modifier, 6.3-8.3 isoelectric point, 0.48-0.62 mobility of monomer and 0.11-0.17 mobility of pentamer. USE:A diagnosticum for Alzheimer's senile dementia and Alzheimer's disease. PREPARATION:For example, a splenic cell which is obtained by immunizing a mouse against amyloid protein separated from autopsy spleen of a patient of human protopathic amyloidosis as an antigen and a mouse myeloma cell are subjected to cell fusion, the formed fused cell is cloned by limiting dilution method into a monoclone and then the monoclonal cell is cultivated to obtain a monoclonal antibody from the supernatant liquid of the culture mixture.

机译：新材料：一种对脑结构中的老年斑具有反应性的单克隆抗体，分子量为160,000-180,000mol.wt。作为单体和800,000-1,000,000mol。重量通过使用十二烷基硫酸钠作为蛋白质改性剂，6.3-8.3等电点，0.48-0.62单体迁移率和0.11-0.17戊二烯迁移率的聚丙烯酰胺凝胶二次电泳法得到五聚体。用途：阿尔茨海默氏症老年痴呆症和阿尔茨海默氏病的诊断用药。制备：例如，通过对小鼠免疫分离的脾细胞，所述脾细胞以抗原分离自人原发性淀粉样变性病患者的尸检脾脏的淀粉样蛋白，并与小鼠骨髓瘤细胞进行细胞融合，通过有限稀释法制成单克隆，然后培养单克隆细胞，从培养混合物的上清液中获得单克隆抗体。

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公开/公告号JPS62267297A

专利类型
公开/公告日1987-11-19

原文格式PDF
申请/专利权人 TOKYO MET GOV SEISHIN IGAKU SOGO KENKYUSHO;
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申请/专利号JP19860109433
发明设计人 ISHII TAKESHI;SHINODA TOMOTAKA;
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申请日1986-05-15
分类号C07K14/195;C07K1/22;C07K14/005;C07K16/00;C12N5/00;C12N5/10;C12N15/00;C12N15/02;C12P21/00;C12P21/08;C12R1/91;G01N33/53;G01N33/577;
国家 JP
入库时间 2022-08-22 07:04:33

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