A cDNA probe recognising the mRNA of the uncoupling protein of mammalian brown adipose tissue Mitochondria, process for its preparation and its application

• 1. 一种识别线粒体粘度的荧光探针及其制备方法与应用 [P] . 中国专利： CN110305055A . 2019-10-08
• 2. 敲入有解偶联蛋白1-荧光素酶基因的非人哺乳动物模型及其构建方法和应用 [P] . 中国专利： CN103947606B . 2015.09.30
• 3. A cDNA probe recognising the mRNA of the uncoupling protein of mammalian brown adipose tissue Mitochondria, process for its preparation and its application [P] . 欧洲知识产权局专利： EP0148686A2 . 1985-07-17

  机译： 识别哺乳动物棕色脂肪线粒体解偶联蛋白mRNA的cDNA探针，制备方法及应用

• 4. Probe of cdna recognizing the mrna of the protein uncoupling the mitochondria of the brown adipose tissue of mammals, application [P] . 法国专利： FR2557137B1 . 1986-05-23

  机译： cdna识别哺乳动物棕色脂肪组织线粒体解偶联蛋白mRNA的探针，应用

• 5. ADNC PROBE RECOGNIZING THE MRNA OF THE MITOCHONDRIA PROTEIN DECOUPLANT OF BROWN FATTY MAMMALIAN TISSUE, METHOD OF PREPARATION AND APPLICATION [P] . 法国专利： FR2557137A1 . 1985-06-28

  机译： ADNC探针识别棕色脂肪哺乳动物组织的线粒体蛋白质十倍体的mRNA，制备方法和应用

• 1. Effects of dietary protein levels and genetic background on growth and carcass performance, serum parameter, enzyme and transcription factor mRNA expression in subcutaneous adipose tissues in swine [C] . W.T.Gu, Z.Q.Liu, W.J.Tang, Progress in functional amino acids and carbohydrates for animal production . 2009
• 2. Wheat mitochondrial orf256-coxl RNA binding proteins and tissue -specific cDNAs. [D] . El Shehawi, Ahmed Mohamed. 2001
• 3. Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine and ovine species. Sudden occurrence of uncoupling protein mRNA during embryogenesis and its disappearance after birth. [O] . L Casteilla, O Champigny, F Bouillaud, 1989
• 4. Rapid increase of mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue Use of a cDNA probe [O] . Ricquier Daniel, Mory Gérard, Bouillaud Frédéric, 1984

• 1. Effect of Acupuncture on Uncoupling Protein 1 Gene Expression for Brown Adipose Tissue of Obese Rats [J] . 刘志诚 ,孙凤岷 ,赵东红 . 中国中西医结合杂志：英文版 . 2003,第3期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Application of restriction display PCR technique in the preparation of cDNA microarray probes [J] . Zhao-Hui Sun ,Wen-Li Ma ,Bao Zhang . 世界胃肠病学杂志：英文版 . 2005,第48期
• 4. ＜＇35＞S标记乙型脑炎cDNA探针的研究及应用 [C] . 肖元朴 ,李正谦 . 中华医学会全国第三届微生物检验学术会议 . 1998
• 5. 特异性寡核苷酸探针检测线粒体DNA点突变在线粒体疾病诊断中的应用 [A] . 高素琴 . 2004