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Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same
Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same
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机译：稳定胆碱酯酶，使用稳定的胆碱酯酶的检测试剂盒以及其制备和使用方法
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摘要
The stability of a cholinesterase (particularly a cholinesterase solution impregnated into a porous material and air-dried) can be improved by: (a) buffering the cholinesterase solution with a zwitterionic buffer, e.g. a buffer having a sulfonic acid group and a protonatable amine group, and, preferably, (b) further drying the impregnated, air-dried porous material under a high vacuum (e.g. 0.01 mm Hg or less) at normal ambient temperatures. The most useful porous materials are sheet-like in nature; that is, they have only two major surfaces. An impregnated, sheet-like material of this invention can be used in a cholinesterase inhibitor detector kit. A typical kit of this type provides a simple means for detecting, inter alia, environmental cholinesterase-inhibiting pollutant, e.g. organophosphorous pesticides and nerve agents.
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