首页> 外国专利> METHOD FOR QUANTITATIVE DETERMINATION OF SERUM PROTEIN IN TURBID SERUM OR PLASMA SAMPLES TENSIDE AGENT FOR SERUM WORK-UP

METHOD FOR QUANTITATIVE DETERMINATION OF SERUM PROTEIN IN TURBID SERUM OR PLASMA SAMPLES TENSIDE AGENT FOR SERUM WORK-UP

机译:血清标本在涡轮血清或血浆样品中的含量测定方法

摘要

The invention relates to a process for the quantitative determination of a serum protein, especially apolipoprotein, in turbid serum or plasma samples by nephelometry, which comprises, after having carried out an immunologic reaction with the corresponding antibody, measuring in the presence of a tenside of the formula CH3-(CH2)m-R-(CH2-CH2-O)n-H (I) in which R is O; NH; CHCH(CH2)xNH; +TR IMAGE Y is H- or CH3-(CH2)q-, m and q, being identical or different; each are an integer of from 6 to 26, preferably 6 to 18; n is an integer of from 7 to 50, preferably 7 to 25; and x is 7 or 8; the tenside being used in a concentration of from 10-3 to 10-1% by volume. Alternatively, the measures can be taken after enzymatic degradation of the lipids and subsequent immunologic reaction with the corresponding antibody in the presence of a nonionic tenside of the formula (I), in which R is as defined above, or IMAGE +TR R' being CH3 or C2H5, Further subjects of the invention are the use of a tenside of the cited formula for serum work-up for quantitative determination of an apolipoprotein, and an agent for serum work-up for quantitative determination for an apolipoprotein, consisting of a mixture of at least one lipid-degrading enzyme and a tenside of the cited formula.
机译:本发明涉及一种通过浊度法定量测定混浊的血清或血浆样品中的血清蛋白,特别是载脂蛋白的方法,该方法包括在与相应抗体进行免疫反应后,在存在的表面活性剂的存在下进行测定。式CH3-(CH2)mR-(CH2-CH2-O)nH(I)其中R为O; NH; CHCH(CH2)xNH; + TR Y是H-或CH3-(CH2)q-,m和q,相同或不同;每个为6至26的整数,优选6至18; n为7至50,优选7至25的整数; x是7或8;表面活性剂的使用浓度为10-3%至10-1%(体积)。或者,可以在脂质进行酶促降解并随后在式(I)的非离子表面活性剂存在下与相应的抗体发生免疫反应之后采取措施,其中R如上定义,或 + TR R作为CH 3或C 2 H 5,本发明的另外的主题是所引用的配方的一个方面在血清后处理中用于定量测定载脂蛋白的用途,以及在血清后处理中用于定量测定载脂蛋白的试剂的用途,包括至少一种脂质降解酶和所引用配方的表面活性剂的混合物。

著录项

  • 公开/公告号NZ190899A

    专利类型

  • 公开/公告日1982-09-07

    原文格式PDF

  • 申请/专利权人 HEUCK C;

    申请/专利号NZ19790190899

  • 发明设计人 HEUCK C;

    申请日1979-07-03

  • 分类号G01N33/536;G01N33/68;G01N33/92;

  • 国家 NZ

  • 入库时间 2022-08-22 13:39:29

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