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Clinically active bovine growth hormone fraction

机译:临床活性牛生长激素分数

摘要

A clinically active component (TBGH-d) of a tryptic digest of bovine growth hormone (BGH) found to be homogeneous by disc electrophoresis and sedimentation equilibrium and having substantially the same molecular weight and amino acid composition as BGH was separated into two fractions, A-I and A-II, by dextran gel filtration in 50% acetic acid. The amino acid analysis of the A-I and A-II fractions accounted for all the amino acids in the TBGH-d. The biological activity of the A-II fraction was found to be about 10-30% of TBGH-d. This fraction, having a molecular weight of about 5000 and an amino acid content or chain length of 38 amino acids, appears to exhibit human growth hormone-like activity. This fraction also appears to have wider applicability than simply being useful for the acceleration of somatic growth in humans. For example, this fraction would appear to have utility in promoting increased function and growth in an impaired liver and in effecting tissue regeneration, such as the promotion of healing of stress ulcers. It would also appear that this fraction has utility in the stimulation of protein synthesis to increase immunological competence and in wound and fracture healing. Also, it would appear that this fraction has utility in non- human applications, e.g., for increasing milk production, for increasing wool production and for accelerating animal growth.
机译:通过圆盘电泳和沉降平衡发现牛生长激素(BGH)胰蛋白酶消化物的临床活性成分(TBGH-d)均质,并且具有与BGH基本上相同的分子量和氨基酸组成,将其分为两部分AI和A-II,通过在50%乙酸中的葡聚糖凝胶过滤。 A-I和A-II部分的氨基酸分析说明了TBGH-d中的所有氨基酸。发现A-II级分的生物活性约为TBGH-d的10-30%。该级分具有约5000的分子量和38个氨基酸的氨基酸含量或链长,似乎表现出人生长激素样活性。除了简单地用于促进人类的体细胞生长之外,该部分似乎还具有更广泛的适用性。例如,该级分似乎在促进受损肝中的功能增强和生长以及影响组织再生,例如促进应激性溃疡的愈合中具有效用。还似乎该部分在刺激蛋白质合成以增加免疫学能力以及伤口和骨折愈合中具有效用。同样,该级分似乎在非人类应用中具有效用,例如用于增加牛奶产量,增加羊毛产量和加速动物生长。

著录项

  • 公开/公告号US4056520A

    专利类型

  • 公开/公告日1977-11-01

    原文格式PDF

  • 申请/专利权人 RESEARCH CORPORATION;

    申请/专利号US19750573902

  • 发明设计人 MARTIN SONENBERG;NOBUYUKI YAMASAKI;

    申请日1975-05-02

  • 分类号C07C103/52;A61K37/00;

  • 国家 US

  • 入库时间 2022-08-22 23:27:55

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