首页> 外国专利> 'A PROCESS FOR THE ISOLATION OF BACTERIOPHAGE GENOME USEFUL AS A TOOL FOR STUDYING BIOLOGICAL, BIOCHEMICAL, PHYSIOLOGICAL AND GENETIC PROPERTIES OF ACTINOMYCETES AND OTHER ORGANISMS'

'A PROCESS FOR THE ISOLATION OF BACTERIOPHAGE GENOME USEFUL AS A TOOL FOR STUDYING BIOLOGICAL, BIOCHEMICAL, PHYSIOLOGICAL AND GENETIC PROPERTIES OF ACTINOMYCETES AND OTHER ORGANISMS'

机译:“用于研究噬菌体和其他生物的生物,生物化学,生理和遗传特性的细菌噬菌体基因组的分离方法”

摘要

This invention relates to a process for the isolation of bacteriophage genome useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which comprises incubating a novel strain of Saccharomonospora having characteristics as herein described in a nutrient medium such as herein described until the autolysis stage is reached, isolating and purifying from the said medium a bacteriophage having a double stranded DNA genome of about 90 KB generated in the lysed culture by conventional methods, then breaking-up the proteinaceous envelop of the said isolated bacteriophage by known methods followed by recovering the bacteriophage genome by conventional precipitation techniques.
机译:本发明涉及一种分离噬菌体基因组的方法,该方法可用作研究放线菌和其他生物的生物学,生化,生理和遗传特性的工具,该方法包括在营养培养基如在此描述直到达到自溶阶段为止,通过常规方法从所述培养基中分离和纯化在裂解培养物中产生的具有约90KB的双链DNA基因组的噬菌体,然后通过以下方法破碎所述分离的噬菌体的蛋白质包膜。已知方法,然后通过常规沉淀技术回收噬菌体基因组。

著录项

  • 公开/公告号IN1998DE03163A

    专利类型

  • 公开/公告日1900-01-01

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN3163/DEL/1998

  • 发明设计人 PUSHPA AGRAWAL;VISHAL SONI;

    申请日1998-10-28

  • 分类号C12N7/02;

  • 国家 IN

  • 入库时间 2022-08-24 17:16:50

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