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'A PROCESS FOR THE ISOLATION OF BACTERIOPHAGE GENOME USEFUL AS A TOOL FOR STUDYING BIOLOGICAL, BIOCHEMICAL, PHYSIOLOGICAL AND GENETIC PROPERTIES OF ACTINOMYCETES AND OTHER ORGANISMS'
'A PROCESS FOR THE ISOLATION OF BACTERIOPHAGE GENOME USEFUL AS A TOOL FOR STUDYING BIOLOGICAL, BIOCHEMICAL, PHYSIOLOGICAL AND GENETIC PROPERTIES OF ACTINOMYCETES AND OTHER ORGANISMS'
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机译:“用于研究噬菌体和其他生物的生物,生物化学,生理和遗传特性的细菌噬菌体基因组的分离方法”
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摘要
This invention relates to a process for the isolation of bacteriophage genome useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which comprises incubating a novel strain of Saccharomonospora having characteristics as herein described in a nutrient medium such as herein described until the autolysis stage is reached, isolating and purifying from the said medium a bacteriophage having a double stranded DNA genome of about 90 KB generated in the lysed culture by conventional methods, then breaking-up the proteinaceous envelop of the said isolated bacteriophage by known methods followed by recovering the bacteriophage genome by conventional precipitation techniques.
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