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METHOD FOR DETERMINING NARCOTIC DRUGS IN HUMAN ORAL FLUID BY IMMUNOCHROMATOGRAPHY
METHOD FOR DETERMINING NARCOTIC DRUGS IN HUMAN ORAL FLUID BY IMMUNOCHROMATOGRAPHY
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机译：免疫层析法测定人口腔液中麻醉药物的方法
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摘要
FIELD: medicine.;SUBSTANCE: invention relates to medicine and describes a method for lateral flow immunochromatography for determination of narcotic drugs in the oral fluid in a person, while the method includes collecting oral fluid by a collector and then applying it to a test strip containing sequentially arranged zones: an At-tag conjugate containing specific antibodies to the drug, labeled with gold; a test containing immobilized conjugates analyte : ovalbumin; and a control containing anti-species antibodies; with the subsequent determination of the validity of the test by observing the presence of a colored band in the control zone, and determining the presence of a drug in the oral fluid by observing the appearance of a colored band in the test zone, and an analyte is used as a conjugate: a protein obtained by the carbodiimide method from the macromolecular carrier ovalbumin, as an analute haptens that are derivatives of narcotic substances (morphine, amphetamine, cocaine and tetrahydrocannabinol), containing a spacer with a carboxyl group with a ratio of the number of moles of hapten per protein molecule equal to 12-15, are used, and as a conjugate of the At-label, monoclonal antibodies to the studied drug antigens are used, obtained by hybridomic technology based on conjugated antigens obtained from the macromolecular carrier of bovine serum albumin and the above haptens, derivatives of narcotic substances. Described is possibility of multianalytic immunochromatographic determination of opiates. ;EFFECT: method provides increased sensitivity, expressiveness and time for saving test results.;1 cl, 8 tbl, 12 ex
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机译：田地：药物。物质：发明涉及药物和描述一种用于测定人中口腔流体中麻醉药物的侧流免疫层析的方法，而该方法包括收集器通过收集器收集口腔流体，然后将其施加到测试条上依次排列的区：用金子标记的药物含有特异性抗体的AT-TAG缀合物;含有固定化缀合物分析物的试验：卵磷酸酯;还有含有抗物质抗体的对照;随后通过观察控制区中的彩带的存在，通过观察测试区中彩带的外观和分析物来确定对照区域中的彩带的存在，通过观察口腔流体中的药物的存在，随后确定测试的有效性。用作缀合物：由碳二亚胺方法从大分子载体卵烧蛋白获得的蛋白质，作为麻醉物质（吗啡，amphetamine，可卡因和四氢尼酚）的衍生物，其含有与羧基的间隔物的衍生物使用相当于12-15的半蛋白质分子的摩尔数，并且作为所研究的药物抗原的缀合物，通过基于从大分子获得的共轭抗原获得的杂交型技术获得牛血清白蛋白的载体和上述哈维特，麻醉药物的衍生物。描述的是鸦片蛋白质的多层免疫层析测定的可能性。 ;效果：方法提供更高的灵敏度，表达和节省测试结果的时间。; 1 cl，8 tbl，12 ex
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