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CELL SORTING USING A HIGH THROUGHPUT FLUORESCENCE FLOW CYTOMETER
CELL SORTING USING A HIGH THROUGHPUT FLUORESCENCE FLOW CYTOMETER
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机译：使用高通量荧光流式细胞仪进行细胞分选
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摘要
In one aspect, illuminating the cell with radiation having at least two optical frequencies shifted from one another by radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and fluorescence Sorting cells in a flow cytometry system comprising processing temporal fluorescence data to arrive at a classification decision regarding the cells without generating an image of the cells (i.e., pixel-to-pixel images) based on the data. A method is disclosed. In other words, although the fluorescence data may contain image data capable of generating a pixel-to-pixel fluorescence intensity map, the method arrives at a classification decision without generating such a map. In some cases, classification decisions may be made with a latency of less than about 100 microseconds. In some embodiments, the method of classifying a cell may have sub-cellular resolution, eg, a classification decision may be based on characteristics of a component of the cell. In some implementations where more than two frequency-shifted optical frequencies are used, a single radiofrequency shift is used to separate the optical frequencies, and in other such implementations, a plurality of different radiofrequency shifts are used.
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