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首页> 外文学位 >Lab-on-a-chip flow cytometer and microfluidic fluorescence activated cell sorter (muFACS) for biomedical applications.
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Lab-on-a-chip flow cytometer and microfluidic fluorescence activated cell sorter (muFACS) for biomedical applications.

机译：芯片实验室流式细胞仪和微流控荧光激活细胞分选仪（muFACS），用于生物医学应用。

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摘要

This dissertation details the development of a portable, low cost lab-on-a-chip flow cytometer and/or fluorescence-activated cell sorter (muFACS), in which microfluidics, micro-acoustics, on-chip optics and electronics are integrated into one tiny polydimethylsiloxane (PDMS) chip creating novel functionalities. This work demonstrates a compact, high-speed sorter, integrated optofluidic waveguides, and a novel color-space-time coding technique for low-cost fluorescence detection.;The microfluidic fluorescence-activated cell sorter employs fast-response piezoelectric actuators in conjunction with a high-speed, low timing jitter closed loop control system ensures high purity sorting of targeted biological samples with single-cell manipulation capabilities. By deflecting the entire sub-nanoliter volume of fluid, the iFACS can sort various biological samples regardless of their physical or chemical properties. A high enrichment factor (>230 fold) is demonstrated at a sorting throughput of greater than 1,000 cells/sec. By integrating the entire sorting system onto the chip, this technology holds great promise to rapidly become competitive with commercial benchtop sorters.;Teflon AF coated optofluidic waveguides demonstrated in this work enhance the coupling efficiency of photons to fluorescent samples, thus increasing sensitivity and permitting multi-spot illumination. The waveguides also enable the space-time coding technology, which works in conjunction with a specially designed spatial filter and the finite-impulse-response (FIR) match filter algorithm in order to further enhance detection sensitivity.;The multi-color fluorescence detection technology with an on-chip color filter waveguide array, known as Color-Space-Time (COST) coding, enables the discrimination of up to 11 fluorescent wavelengths using a single photodetector. This novel technology holds great promise for compactness by fundamentally altering the scaling rule; that is the number of bulky optical components required for detection will no longer scale linearly with the number of detection parameters. In this way, the technique can significantly lower the cost and the volume of the whole system in addition to miniaturization of the device.;Such a low-cost, compact, portable flow cytometer and muFACS system can be readily afforded by individual clinics and research labs, providing point-of-care diagnosis and analysis. The development of the microfluidic flow cytometer/muFACS can improve global quality of life; and while the technology is not fully ready today, great strides have been made towards achieving this goal, as demonstrated in this dissertation.
机译：本论文详细介绍了便携式，低成本芯片实验室流式细胞仪和/或荧光激活细胞分选仪（muFACS）的开发，其中微流体，微声学，片上光学器件和电子器件集成在一起微小的聚二甲基硅氧烷（PDMS）芯片可创建新颖的功能。这项工作展示了一个紧凑的高速分选器，集成的光流体波导以及一种用于低成本荧光检测的新颖的色-空-时编码技术。该微流体荧光激活细胞分选器采用了快速响应的压电驱动器，并结合了一个高速，低时序抖动闭环控制系统可确保具有单细胞操作能力的目标生物样品的高纯度分选。通过偏转整个亚纳升以下的液体体积，iFACS可以对各种生物样品进行分类，而不管其物理或化学特性如何。在大于1,000个细胞/秒的分选通量下证明了高富集因子（> 230倍）。通过将整个分选系统集成到芯片上，这项技术有望与商用台式分选机迅速竞争。；这项工作中展示的特氟龙AF涂层光流体波导提高了光子与荧光样品的耦合效率，从而提高了灵敏度并允许多点照明。波导还支持空时编码技术，该技术与专门设计的空间滤波器和有限冲激响应（FIR）匹配滤波器算法结合使用，以进一步提高检测灵敏度。;多色荧光检测技术芯片上的彩色滤光片波导阵列（称为色空时间（COST）编码）可以使用单个光电探测器识别多达11个荧光波长。通过从根本上改变缩放比例规则，这项新颖的技术有望实现紧凑性。也就是说，检测所需的庞大光学组件的数量将不再与检测参数的数量成线性比例关系。这样，除了使设备小型化外，该技术还可以显着降低整个系统的成本和体积。这样的低成本，紧凑，便携式流式细胞仪和muFACS系统可以由各个诊所和研究机构轻松提供实验室，提供即时诊断和分析。微流式细胞仪/ muFACS的发展可以改善全球生活质量；正如今天的论文所展示的，尽管目前技术还没有完全准备就绪，但是在实现这一目标方面已经取得了长足的进步。

著录项

  • 作者

    Cho, Sung Hwan.;

  • 作者单位

    University of California, San Diego.;

  • 授予单位 University of California, San Diego.;
  • 学科 Engineering Biomedical.;Engineering Electronics and Electrical.;Engineering Materials Science.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 132 p.
  • 总页数 132
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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