Method for Establishing Submerged Vegetation System in Eutrophic Water Body

• 1. METHOD OF EUTROPHIC WATER BODY RESTORATION BASED ON ECOSYSTEM MODEL AND BIOMANIPULATION TECHNOLOGY [P] . AU2020101936A4 . 2020-10-01

  机译： 基于生态系统模型和生物操纵技术的富营养化水体修复方法

• 2. EUTROPHIC WATER BODY RESTORATION METHOD BASED ON ECOSYSTEM MODEL AND BIOMANIPULATION TECHNOLOGY [P] . 世界知识产权组织专利： WO2019210551A1 . 2019-11-07

  机译： 基于生态系统模型和生物操纵技术的富营养体修复方法

• 3. Method and device useful for restoring eutrophic waters using aluminum or iron salts in solid or liquid form as precipitant, which is in the form of a suspension produced with sea water in venturi nozzle-mixing system [P] . 德国专利： DE102012024993A1 . 2014-06-26

  机译： 用于以固体或液体形式的铝盐或铁盐作为沉淀剂来恢复富营养化水的方法和设备，其形式为在文丘里喷嘴混合系统中用海水产生的悬浮液

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Strategy amp;Techniques for Restoration of Healthy Aquaecosystem from Toxic Super Eutrophic Water Body [J] . Peimin Pu ,Jiangping Pu . 地球科学和环境保护期刊（英文） . 2015,第6期
• 3. Research of the Method of Establishing Evaluation Quota System for ATIS Projects [C] . Wu Zhizhou ,Yang Xiaoguang ,Sun Jian . 第五届交通运输领域国际学术会议 . 2005
• 4. Exploring the Meaning of Water: A Case Study of Qujing and Water System in Quzhou [A] . 陈艳 . 2012