首页> 外国专利> Three-dimensional cultured epidermis model and its manufacturing method, and how to use the three-dimensional cultured epidermis model

Three-dimensional cultured epidermis model and its manufacturing method, and how to use the three-dimensional cultured epidermis model

机译:三维培养表皮模型及其制作方法,以及如何使用三维培养表皮模型

摘要

To provide a three-dimensional cultured epidermis model that allows epidermal cells alive to be observed real-time from multiple directions and the state of the epidermal cells to be more accurately evaluated.SOLUTION: According to the present invention, there is provide a three-dimensional culture epidermis model in which cells or organelles of each layer constituting the epidermis are three-dimensionally visualized with a fluorescent substance. According to the present invention, there is also provided a method for producing three-dimensional cultured epidermis model, the method comprising the steps of staining keratinocytes with a fluorescent dye or introducing a fluorescent protein expression vector into the keratinocytes, and three-dimensionally culturing the obtained keratinocytes.SELECTED DRAWING: None
机译:提供一种三维培养表皮模型,可以从多个方向实时观察活的表皮细胞,并更准确地评估表皮细胞的状态。解决方案:根据本发明,提供了一种三维培养表皮模型,其中构成表皮的每一层的细胞或细胞器用荧光物质三维可视化。根据本发明,还提供了一种制备三维培养表皮模型的方法,该方法包括用荧光染料染色角质形成细胞或将荧光蛋白表达载体引入角质形成细胞,以及三维培养获得的角质形成细胞的步骤。所选图形:无

著录项

  • 公开/公告号JP7041977B2

    专利类型

  • 公开/公告日2022-03-25

    原文格式PDF

  • 申请/专利权人 日本メナード化粧品株式会社;

    申请/专利号JP20200191003

  • 发明设计人 井上 悠;長谷川 靖司;

    申请日2020-11-17

  • 分类号C12N5/10;C12Q1/02;C12N15/12;C12N15/63;C12M3;C12M1/34;

  • 国家 JP

  • 入库时间 2022-08-25 00:14:41

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