首页> 外国专利> Lachnospiraceae bacterium ND2006 CAS12A mutant genes and polypeptides encoded by them

Lachnospiraceae bacterium ND2006 CAS12A mutant genes and polypeptides encoded by them

机译：Lachnospiraceae细菌ND2006 CAS12A突变基因及其编码的多肽

页面导航

摘要
著录项
相似文献

摘要

The present invention relates to nucleic acids and proteins of mutant Lachnospiraceae bacteria ND2006 (Lb) Cas12a for use in the CRISPR / Cas12a endonuclease system, and how to use them. In particular, the invention relates to an isolated mutant LbCas12a protein that is active in the CRISPR / Cas12a endonuclease system. The invention also includes an isolated nucleic acid encoding a variant LbCas12a protein, a ribonucleoprotein complex, and a CRISPR / Cas12a endonuclease system with a variant LbCas12a protein.

机译：本发明涉及在CRISPR/Cas12a内切酶系统中使用的突变Lachnospiraceae细菌ND2006（Lb）Cas12a的核酸和蛋白质，以及如何使用它们。具体而言，本发明涉及在CRISPR/Cas12a核酸内切酶系统中具有活性的分离突变体LbCas12a蛋白质。本发明还包括编码变体LbCas12a蛋白质的分离核酸、核糖核蛋白复合物和具有变体LbCas12a蛋白质的CRISPR/Cas12a内切酶系统。

著录项

公开/公告号JP2022523189A

专利类型
公开/公告日2022-04-21

原文格式PDF
申请/专利权人インテグレーティッドディーエヌエイテクノロジーズインコーポレーティッド;
展开▼

申请/专利号JP20210548687
发明设计人ボードワンサラ・フランツ;コリンウッドマイケル・アレン;バクルスカスクリストファー・アンソニー;
展开▼

申请日2020-02-21
分类号C12N15/09;C12N9/22;C12N15/31;C12N15/55;C12N15/63;
国家 JP
入库时间 2022-08-25 00:41:04

相似文献

专利
外文文献
中文文献

1. Safety, immunogenicity, and cross-species protection of a plasmid DNA encoding Plasmodium falciparum SERA5 polypeptide, microbial epitopes and chemokine genes in mice and olive baboons [J] . Nyamongo Onkoba, Ruth M.Mumo, Horace Ochanda, 生物医学研究杂志（英文版） . 2017,第004期

2. Safety,immunogenicity,and cross-species protection of a plasmid DNA encoding Plasmodium falciparum SERA5 polypeptide,microbial epitopes and chemokine genes in mice and olive baboons [J] . Nyamongo Onkoba, Ruth M.Mumo, Horace Ochanda, 生物医学研究杂志：英文版 . 2017,第004期

3. Characterization and Expression Profiling of Genes Encoding the Gibberellin3-oxidase in Dasypyrum villosum Dwarf Mutant [J] . Peng CAI, Zaijun YANG, Zhengsong PENG, 农业科学与技术（英文版） . 2017,第009期

4. EFFECTS OF A SYNTHETIC POLYPEPTIDE ENCODED BY THE p14-6,A cDNA CLONE WITH ANTIONCOGENE ACTIVITY,ON MALIGNANT TRANSFORMED DT CELLS [J] . 中国癌症研究（英文版） . 1992,第001期

5. Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1 (B 870) [J] . Raiger-Iustman LJ, Kerber NL, Pucheu NL, Archives of microbiology . 2006,第6期

6. Nucleotide Sequence of the psbP Gene Encoding Precursor of 23-kDa Polypeptide of Oxygen-Evolving Complex in Arabidopsis thaliana and its Expression in the Wild-Type and a Constitutively Photomorphogenic Mutant [J] . Akhilesh K. Tyagi, Anju Kochhar, Jitendra P. Khurana DNA research: an international journal for rapid publication of reports on genes and genomes . 1996,第5期

7. The moxFG region encodes four polypeptides in the methanol-oxidizing bacterium Methylobacterium sp. strain AM1. [J] . D J Anderson, M E Lidstrom Journal of bacteriology . 1988,第5期

8. DETECTION OF THE GENE ENCODING THE SMALL SUBUNIT OF THE CO DEHYDROGENASE ENZYME IN THE H_2-EVOLVING BACTERIUM RUBRIVIVAX GELATINOSUS CBS [C] . Adrienne Kish, David Levin Canadian Hydrogen Conference: Building the Hydrogen Economy v.2; 20010617-20010620; Victoria; CA . 2001

9. Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II [D] . Chung, Kyu Hwan 1994

10. The moxFG region encodes four polypeptides in the methanol-oxidizing bacterium Methylobacterium sp. strain AM1. [O] . D J Anderson, M E Lidstrom 1988

11. Nucleotide Sequence of the psbP Gene Encoding Precursor of 23-kDa Polypeptide of Oxygen-Evolving Complex in Arabidopsis thaliana and its Expression in the Wild-Type and a Constitutively Photomorphogenic Mutant [O] . Anju Kochhar, Jitendra P. Khurana, Akhilesh K. Tyagi 1996

12. Secretory Polypeptides Encoded in Balbiani Ring Genes [R] . Case, S. T. 1992

1. Replication and gene expression of mutant hepatitis B virus in a transgenic mouse containing the complete viral genome with mutant sgene [J] . Jun-HuiGe ,Le-ZhiZhang ,Jian-XiuLi . 世界胃肠病学杂志：英文版 . 2004,第21期

2. Molecular cloning and characterization of a gene encoding methionine aminopeptidase 2 of Schistosoma japonicum [C] . Jinbiao Peng ,Hongxiao Han ,Yang Hong . 上海市畜牧兽医学会2011年学术年会 . 2011

3. A.Characterization of two novel Metallic compounds on P53-E6 appoptotic signaling pathway in Human Cervical Cancer cells.B.Cancer gene therapy using a novel survivin mutant D53A adenovirus. [A] . Naser uddin Hoti . 2004